Prof. (Dr.) Pradyumna Misra , Dr. Gaurav Jain , Dr. Preeti Shukla , Dr. Sonali Verma , Dr. Shatakshi Rastogi 1. Vice-Principal, Professor and Head of Department, Department of Conservative Dentistry and Endodontics, Saraswati Dental College, Lucknow – 227105 Uttar Pradesh, India Corresponding Author: 2. Dr. Gaurav Jain Associate Professor, 3,4,5 Department of Conservative Dentistry and Endodontics, Saraswati Dental College & Hospital, Department of Conservative Dentistry and Endodontics, Saraswati Dental College, Lucknow – 227105 Uttar Pradesh, India.
Abstract :-
Background :
Main aim of endodontic treatment is to make the root canal free from microorganisms and
their byproducts. Medicinal herbs, showing anti-microbial property, bio-friendly and lower side
effects, can be used in conjunction with sealer during obturation to enhance the success rate of
treatment. Hence, the aim of the present in-vitro study was to compare the anti-microbial activity
of AH26 sealer when mixed with Amoxicillin, M. longifolia and Asafetida extract on
Enterococcus faecalis. Materials & Method :
In this study 3000 µl of AH26 sealer was mixed and prepared according to manufacturer’s
instructions and divided into four sections for experimental groups. First group, contained only
AH26 sealer. In second, third and fourth groups, Amoxicillin powder, M. longifolia extract and
Asafetida powder extract was added at 10% of sealer total weight to AH26, respectively. Positive
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and negative control groups contained bacteria or cultivation environment, respectively.
Subsequently, after 24 hours incubation, 10µl of the available solution in each tube were
transferred to BHI Agar for counting the bacteria colonies. Data were analyzed using ANOVA,
post hoc Tukey tests. Statistical significance was set at p<0.05.
Result :
There was not statistically significant (p=0.64) result in AH26 (Group 1) and AH26 + M.
longifolia extract (Group 3) while this difference was highly significant for AH26+ Amoxicillin
(Group 2) and AH26 + Asafetida extract (Group 4). However, the lowest mean of log10 CFU/ml is
for Group 1- AH26 (2.23) and the highest mean is for Group 2- AH26+ Amoxicillin (5.42).
Conclusion :
It is also concluded that anti-microbial activity of herbal extract (Asafetida) might be
compared to those of antibiotics, and when taking into account the likelihood of antibiotic side
effects, this herbal extract can be a good alternative.
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